Wait for the note to settle.
Pick once, then listen. If the attack begins sharp, tune from the stable sustain instead of the first transient.
Pitch, cents, reference tones.
Start the microphone, play one clear note, and tune from the stable sustain after the attack settles.
Microphone is off. Reference tones remain available.
Play the reference for an open string, listen, then tune. Use the display to confirm what your ear already heard.
Pick once, then listen. If the attack begins sharp, tune from the stable sustain instead of the first transient.
When possible, flatten the string slightly and tune upward. It helps the string seat predictably at the nut and post.
After open strings, play the chords and positions you are about to use. Temperature and fretting pressure matter.